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Basement Membrane beneath Serous Mesothelial Cells Contains α1(IV), α2(IV), α5(IV), and α6(IV) Chains of Type IV Collagen Demonstrated by Chain-specific Monoclonal Antibodies Basement Membrane beneath Serous Mesothelial Cells Contains α1(IV), α2(IV), α5(IV), and α6(IV) Chains of Type IV Collagen Demonstrated by Chain-specific Monoclonal Antibodies Naito, Ichiro Matsubara, Tatsuya Takahashi, Chieko Ohmori, Hiroyuki Sado, Yoshikazu Ninomiya, Yoshifumi Okigaki, Tohru IV型コラーゲン 基底膜 漿膜 腹膜 腹膜透析 Collagen type IV basement membranse serous membrane peritoneum CAPD P(論文) Serous membrane (SM) covers inner surface of abdominal, thoracic and pericardial cavities, aswell as outer surface of organs inside the cavities. It consists of surface mesothelial cells andloose connective tissue. Between them, a thim layer of basement membrane (BM) is located. Type IVcollagen is major constituent of BM, and consists of 6 different a(IV) chains, a1(IV) through a6(IV).Chain-specific functions are assumed by a chain-specific manner of localization. The a(IV) chaincomposition of skin, covering outer surface of the body, is demonstrated to have a1(IV), a2(IV), a5(IV),and a6(IV) chains, whereas that of SM, covering inner surface of the body, is yet to be analyzed. Abdominal wall, small intestine, thoracic wall, lung, pericardium and epicardium of humanmaterials were used in this study. Chain-specific monoclonal antibodies (mAbs) used were H11(for a1), H22 (for a2), H31 (fo a3), H43 (for a4), H53 (for a5) and H63 (for a6). Fresh frozen sectionswere stained with indirect immunofluorescence staining using the mAbs. Four out of six a(IV) chains, a1, a2, a5 and a6, were demonstrated in BM beneath themesothelial cells of all types of SMs, whereas only capillary BM consisted of a1 and a2. Besides,epicardial SM expressed a3 and a4 moderately as extra components. The a(IV) chain composition was same as those of epidermal skin BM. Therefore, these a(IV)chains are designated to be essential for BM covering inside and outside of the body. 2000-12-12 eng departmental bulletin paper https://kinwu.repo.nii.ac.jp/records/96 AA11887920 近畿福祉大学紀要 1 20 25 https://kinwu.repo.nii.ac.jp/record/96/files/KJ00004728796.pdf application/pdf 6.2 MB 2000-12-12